T-cell Receptor Gene Rearrangements for Leukemia

Test Includes:

DNA probe to the T-cell receptor beta-chain constant region.

Special Instructions:

Whole blood, bone marrow, lymph node, or other tissue.

Specimen:

7 mL whole blood or 2 cc bone marrow or 0.5-1 g tissue.

Volume:

3 mL whole blood.

Container:

Whole blood or bone marrow in lavender-stopper (EDTA) tubes, lymph node or tissue in lymph node transport bottle.

Collection:

The specimen should arrive in the laboratory within 48 hours of collection. Please state the date and time of collection on the test request form.

Storage:

Maintain specimen at room temperature.

Causes for Rejection:

Specimen frozen; specimen in formalin or another fixative; clotted specimen; hemolysis; blood more than 72 hours old; bone marrow aspirates older than 5 days.

Use:

Determine the clonality and lineage of suspected neoplastic lymphoid cell populations by detecting gene rearrangements that occur normally during the maturation of lymphoid cells into T-lymphocytes.